Phospho-sting ser366

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August undefined, 2024

WebPhospho-STING (Ser366) one-plate assay protocol Detection of Phosphorylated STING (Ser366) with HTRF reagents can be performed in a single plate used for culturing, …

Phospho-STING (Ser366) Polyclonal Antibody Invitrogen PA5 …

WebAfter TBK1 phosphorylates STING at residue S366, phospho-STING forms a platform at the carboxy-terminal tail (CTT) for recruitment of interferon regulatory factor 3 (IRF3) 14 . IRF3 is then ... WebMay 19, 2024 · Load the lysate samples and run a 10% SDS-PAGE gel. Set up a mini gel tank and fill it with 1× running buffer prepared from 10× running buffer. Position the gel inside the tank and load the sample in each well. Run the gel at 200 V until the blue dye front is out. crystal suncatchers for windows prism

sting Primary Antibodies - Thermo Fisher Scientific

WebThe Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor ® 647 Conjugate) Antibody was generated using stimulator of interferon response cGAMP interactor 1, and STING1 as the antigen. It reacts with Human. This antibody has been shown to work in applications such as: Flow Cytometry, Immunocytochemistry, and Immunofluorescence. WebMouse Phospho-STING (Ser365) one-plate assay protocol Detection of Mouse Phosphorylated STING (Ser365) with HTRF reagents can be performed in a single plate used for culturing, stimulation, and lysis. No washing steps are required. This HTS designed protocol enables miniaturization while maintaining robust HTRF quality. Web(50907) Phospho-STING (Ser366) (E9A9K) Rabbit mAb Sign in or register to save this reagent to your favourites Supplier Cell Signaling Technology Host Rabbit Type Primary Clonality Recombinant (E9A9K) Recombinant Yes Target Stimulator of interferon genes protein UniProt Q86WV6 - STING_HUMAN Gene STING1 Modification Phospho (Ser366) crystal sun catchers craft

Phospho-STING (Ser366) Antibody Cell Signaling …

WebThe Mouse Phospho-STING (Ser365) assay measures mouse STING when phosphorylated at Ser365. Unlike Western Blot, the assay is entirely plate-based and does not require gels, … WebJul 7, 2024 · To initiate signaling, serine 365 (S365) in the C-terminal tail (CTT) of STING is phosphorylated, leading to induction of type I interferons (IFNs). Additionally, evolutionary … dynamic bones vrchat pluginWebPhospho-STING (Ser366) (D7C3S) Rabbit mAb recognizes endogenous levels of STING protein only when phosphorylated at Ser366. Species Reactivity: Human Source / … dynamic bone unity asset free

"WebJun 3, 2024 · STING-GFP expression was similar in control and VPS13C KO cells ( Fig. S4 A ), and phospho-STING (Ser366) and phospho-TBK1 (Ser172) remained elevated in the VPS13C KO cells ( Fig. S4, B and C ). In WT cells, STING-GFP was almost exclusively localized to the ER, as expected ( Fig. 5, A and B ). " - Phospho-sting ser366

Phospho-sting ser366

sting Primary Antibodies - Thermo Fisher Scientific

WebThe Total STING cellular assay monitors total STING, and can be used as a normalization assay with our phospho-STING kit. This kit is compatible with the buffers from the phospho-STING kit, so the same lysate can be used for analyses of both the phosphorylated and the total protein populations. Following pathogen infection and binding of dsDNA ... WebThe Phospho-STING (Ser366) (D8K6H) Rabbit mAb (Alexa Fluor ® 647 Conjugate) Antibody was generated using stimulator of interferon response cGAMP interactor 1, and STING1 …

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WebELISA Kits detecting STING can be used to research antiviral response and innate immune response against intracellular pathogen. or to study infectious diseases. STING, also known as Endoplasmic reticulum interferon stimulator (ERIS), is a symbol of Stimulator of interferon genes protein. Your search returned 17 STING ELISA Kit across 4 suppliers. WebThe scaffolding function of STING and other adaptors may explain why IRF3 is activated only in a subset of signaling pathways that activate TBK1. ... IRF3 also bound to phospho-TBK1 [TRAF family member-associated NFκB activator (TANK)-binding ... Further, Ser366 and Leu374 of STING, but not Ser358, are required for phosphorylation of IRF3 ...

WebPhospho-STING (Ser366) Antibody (PA5-117276) in WB Western blot analysis of extracts of THP-1 cells using Phospho-STING (Ser366) Polyclonal Antibody ( Product # PA5-117276 … WebThe Phospho-STING (Ser366) (D8K6H) Rabbit mAb Antibody was generated using stimulator of interferon response cGAMP interactor 1, and STING1 as the antigen. It …

WebMar 25, 2024 · Storage : -20°C. Description : STING is a 379 amino acid containing signaling protein that acts as an important component of innate immune signaling. This signaling protein is able to activate both NF-kappa-B and IRF3 transcription pathways to induce expression of type I interferon (IFN-alpha and IFN-beta) and exert a potent anti-viral state ... WebPhosphorylation by TBK1 leads to activation and production of IFN-beta. Following cyclic nucleotide (c-di-GMP or cGAMP)-binding, activation and translocation from the …

Web(85735) Phospho-STING (Ser366) Antibody This product has been discontinued by Cell Signaling Technology, but remains here on CiteAb for record purposes. Sign in or register to save this reagent to your favourites Supplier Cell Signaling Technology Host Rabbit Type Primary Clonality Polyclonal Conjugate Unconjugated Target

Web(A) At 72 hours post-treatment protein was extracted for immunoblot analysis of phospho-TBK1 (Ser172), total TBK1, phospho-STING (Ser366 in human and Ser365 in murine) and total STING expression with vinculin as loading control. Numbers below the blots represent phosphorylated protein levels quantified by densitometric analysis. dynamic bone unity freeWebInvitrogen Anti-Phospho-STING (Ser366) Polyclonal, Catalog # PA5-105674. Tested in Western Blot (WB), Immunocytochemistry (ICC/IF) and … dynamic bones vs physbonesWeb64 sting Primary Antibodies: Thermo Fisher antibodies are validated for applications including western blotting, immunocytochemistry, flow cytometry, and chromatin immunoprecipitation. ... Phospho-STING (Ser366) Polyclonal Antibody. 1 Reference 1 Reference 6 figures. Target . Human Mouse Rat. Details. Rabbit Polyclonal. Application. … dynamic bone unity package freeWebThe Human Monocyte STING Activation Flow Cytometry Panel includes antibodies targeting phospho-STING (Ser366) and phenotyping markers to enable observation of STING … dynamic bone unity assetWebPolyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser366 of human STING protein. Antibodies are … dynamic bone unity 使い方WebPhospho-IRF-3 (Ser396) (D6O1M) Rabbit mAb (upper) or IRF-3 (D6I4C) XP® Rabbit mAb #11904 (lower). STING (D2P2F) Rabbit mAb #13647: IHC analysis of paraffin-embedded human colon carcinoma using STING (D2P2F) Rabbit mAb. Phospho-STING (Ser366) (D8K6H) Rabbit mAb #40818: Confocal IF analysis of THP-1 cells differentiated dynamic bone unity packageWebThe Human Monocyte STING Activation Flow Cytometry Panel includes antibodies targeting phospho-STING (Ser366) and phenotyping markers to enable observation of STING activation in human monocyte populations among peripheral blood mononuclear cells (PBMCs). CD45 is a pan leukocyte marker. Monocytes and dendritic cells (DCs) are … dynamic bookkeeping