WebNov 13, 2011 · The FET family proteins FUS, EWSR1 and TAF15 are RNA-binding proteins with diverse nuclear functions. PAR-CLIP analyses now reveal the genome-wide RNA targets of all three human FET proteins and ... WebNational Center for Biotechnology Information
Transcriptome-wide Identification of RNA-Binding ... - ScienceDirect
WebDec 27, 2011 · PAR-CLIP PROTOCOL. The Application of Photoactivatable Ribonucleosides for RNA–Protein Crosslinking. As an alternative to the crosslinking of natural nucleic acids with short-wavelength UV light, modification of RNA with photoactivatable nucleoside analogs has been shown to increase the efficiency of nucleic … http://docs.abcam.com/pdf/protocols/CLIP-Protocol.pdf dr seth rials
PAR-CLIP: A Method for Transcriptome-Wide Identification of
WebMay 24, 2024 · To investigate whether EFs interact with RNA in vivo, we used photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation (PAR-CLIP) (Hafner et al., 2010), a method that detects and maps direct protein-RNA interactions without chemical crosslinkers.We applied our recently optimized PAR-CLIP protocol … WebProtocol for RIP-Seq or CLIP-Seq Does anyone have a protocol for pulling down protein associated RNA? The idea is to sequence and identify RNA species associated to a specific RNA binding... WebPAR-CLIP (Photoactivatable Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation): a step-by-step protocol to the transcriptome-wide identification of … colorado state university faculty directory