WebAug 10, 2024 · In HDX assays, NDP52 and ULK1 complex are at 16 and 2 μM concentration respectively, so as to saturate binding and ensure we are studying the bound form. In the GUV assays, the ULK1 complex is at 100 nM concentration, to avoid saturating the imaging and lipid binding in the absence of activation. The destabilization of the MR by NDP52 in … WebThe cluster analysis based on the hydrogen deuterium exchange (HDX) assay data reveals a unique pattern when phytoestrogens and mycoestrogens bind with ERα LBD compared to that of estradiol and ...

Mapping binding epitopes of monoclonal antibodies targeting

WebOverview. Hydrogen deuterium exchange (HDX) mass spectrometry (MS) is a powerful tool for studying the dynamics of higher order structure of protein-based therapeutics. The rate of hydrogen-to-deuterium exchange within the amide hydrogen on the backbone of biotherapeutics provides solvent accessibility information, and thus protein structure ... WebSimoa® Assay Kits Ready-to-use Simoa® kits contain all the reagents necessary to run your assay on the HD-1, HD-X , SR-X or SP-X platforms. Our broad portfolio of available kits spans multiple therapeutic areas including cardiology, infectious disease, inflammation, neurology, and oncology. green costume for women

Hydrogen/Deuterium Exchange Mass Spectrometry with …

WebFeb 16, 2014 · HDX assay. The USP1–UAF1 complex was pre-incubated with 20 μM ML323 on ice for 30 min. Hydrogen-deuterium exchanges on proteins were initiated by diluting each sample fivefold in deuterated ... WebThe system is tailored to address the wide variety of protein sample preparation workflows used in proteomics and biotherapeutic drug discovery and development. Automated … WebMay 12, 2014 · Unlike the HDX assays, however, hydroxyl radical-mediated modification probes exposure of amino acid side chains rather than backbone amide hydrogens. In these assays, the SEM1(86–107) monomers and fibrils were exposed to a 266 nm laser in the presence of hydrogen peroxide to induce hydroxyl radical-mediated modification. flow vacatures