WebNov 2, 2024 · Breier, A. D. Grossman, Whole-genome analysis of the chromosome partitioning and sporulation protein Spo0J (ParB) reveals spreading and origin - distal … WebOct 13, 2001 · A pair of genes designated parA and parB are encoded by many low copy number plasmids and bacterial chromosomes.They work with one or more cis-acting sites termed centromere-like sequences to ensure better than random predivisional partitioning of the DNA molecule that encodes them.The centromere-like sequences nucleate …
Characterization of a prokaryotic SMC protein involved in …
WebJul 7, 2008 · It is envisioned that the partitioning apparatus must consist of elements that are analogous to those involved in chromosome segregation in eukaryotic cells: a centromere binding protein, a mechanism to move the chromosomes towards the cell poles and, possibly, a mechanism of condensing the nascent chromosomes. WebIt is also independent of chromosome partitioning proteins Spo0J and Soj, suggesting the existence of a novel mechanism controlling chromosome structure. Thus, our results demonstrate that, during sporulation, two separable events prepare B. subtilis for asymmetric cell division: the relocation of cell division sites to the cell poles and the ... flag town
Plasmid partitioning and the spreading of P1 partition protein ParB ...
CPS were prepared as previously described10. Briefly, S. pneumoniae cultures were grown until OD550 = 0.3, washed once with PBS and resuspended in buffer A (50 nM Tris-HCl, pH 7.4, 20% sucrose and 50 nM MgSO4). The solution was then supplemented with 400 units of mutanolysin and 6 µg µl−1 … See more The strains used in this study are listed in Supplementary Table 1. S. pneumoniae R800, D39∆cps29and D39 and derivatives were cultivated at 37 … See more Gene modifications (gfp, mkate2 and flag fusions, knockout and domain deletion) in S. pneumoniae were achieved by homologous … See more For co-immunoprecipitation, cultures of S. pneumoniae cells were grown at 37 °C in C+Y medium until OD550 = 0.3. Cell pellets were incubated at 30 °C for 30 min in buffer A (0.1 M … See more Purification of the chimaera 6His-CpsC/D and ParB-6His was performed as described previously10. To purify RocS-∆AH-6His, E. coli BL21 strains were used and cultured at 37 °C … See more WebSep 6, 2024 · The protein binds the chromosome origin of replication (Cori) and has higher-affinity binding to mutated Cori-DNA that increases Cori-plasmid replication in vivo. gapR gene expression is essential for normal rapid growth and sufficient GapR levels are required for the correct timing of chromosome replication. WebOur results demonstrate that the B. subtilis Smc protein, like its eukaryotic counterpart, plays an important role in chromosome structure and partitioning. Keywords. Bacillus subtilis … flagtown post office nj