WebOct 10, 2024 · I used the v1 pbmc dataset (Cell Ranger 1.1.0 from 10X genomics), which is the combination of these 10 pure types (CD14+ Monocytes, CD19+ B cells, CD34+ cells, CD4+ Helper T cells, CD4+/CD25+ Regulatory T cells, CD4+/CD45RA+/CD25− Naive T cells, CD4+/CD45RO+ Memory T cells,CD56+ Natural killer cells, CD8+ Cytotoxic T cells … WebApr 18, 2024 · 构建拟时间轨迹一共分为三步,其中第一步选择较多,二三步基本一致:. 第一步:确定合适的基因(这里仅针对前两种进行说明);. · 简单地比较过程开始时和结束时收集的细胞,找出差异表达的基因;. · 基于不同的cluster找差异基因(官网推荐使用的 ...
"Error: is(object = cds, class2 = "CellDataSet") is not TRUE ... - Github
Webcds <- orderCells (cds) #⚠️使用root_state参数可以设置拟时间轴的根,如下面的拟时间着色图中可以看出,左边是根。根据state图可以看出,根是State1,若要想把另一端设为根,可以按如下操作 #cds <- orderCells (cds, root_state = 5) #把State5设成拟时间轴的起始 … WebMar 29, 2024 · # -----进行降维----- cds <- reduceDimension(cds, max_components = 2, num_dim = 6, reduction_method = 'tSNE', verbose = T) # -----进行聚类----- # (这里先设置num_clusters,不一定最后真就分成5群;我们这里设置5,最后只能得到4群;如果设成4,结果就得到3群) cds <- clusterCells(cds, num_clusters = 5) # Distance cutoff … ikea bench storage outdoor
monocle2 使用笔记 - 简书
WebDec 19, 2024 · where wt_filtered_cds is a valid CellDataSet object that successfully produces results for other analyses. It looks there are two warnings stacked together. I am unsure what might be causing these warnings, but it could easily change results if the function is considering different input than I expect, for example. The learned cell … WebSep 14, 2024 · 知乎,中文互联网高质量的问答社区和创作者聚集的原创内容平台,于 2011 年 1 月正式上线,以「让人们更好的分享知识、经验和见解,找到自己的解答」为品牌使命。知乎凭借认真、专业、友善的社区氛围、独特的产品机制以及结构化和易获得的优质内容,聚集了中文互联网科技、商业、影视 ... WebNov 14, 2024 · aggregate_gene_expression: Creates a matrix with aggregated expression values for... align_cds: Align cells from different groups within a cds align_transform: … ikea bench velcro cushion